| 质粒名称: | pmGFP-P2A-K(AAG)20-P2A-RFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 73344 |
| 存储实验室: | Bob Goldstein |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 (modified) |
| 载体出品公司: | Clontech |
| 空载体大小: | 2600bp |
| 完整质粒大小: | 11900bp |
| 载体类型: | Worm Expression, Cre/Lox, CRISPR |
| 筛选标记: | Hygromycin ; sqt-1(d) (worm phenotypic marker) |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Turbo |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Pmyo-2::GFP + SEC |
| 物种来源: | C. elegans (nematode), Synthetic |
| 插入大小: | 7900 bp |
| 启动子: | myo-2 |
| 标签/融合蛋白: | GFP |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | M13 Forward (tgtaaaacgacggccagt) |
| 3’测序引物 | M13 Reverse (caggaaacagctatgaccatg) |
|
文献引用方法 |
|
| 材料和方法部分: | pDD317 (Pmyo-2::GFP + SEC) was a gift from Bob Goldstein (Addgene plasmid # 73344 ; http://n2t.net/addgene:73344 ; RRID:Addgene_73344) |
| 参考文献部分: | LEA motifs promote desiccation tolerance in vivo. Hibshman JD, Goldstein B. BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0. 10.1186/s12915-021-01176-0 PubMed 34903234 |
买家导航
pDD317 (Pmyo-2::GFP + SEC)载体质粒基本信息
pDD317 (Pmyo-2::GFP + SEC)质粒图谱载体图谱和pDD317 (Pmyo-2::GFP + SEC)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pDD317 (Pmyo-2::GFP + SEC)质粒载体简介
pDD317 (Pmyo-2::GFP + SEC)质粒序列载体序列
LOCUS pDD317 (Pmyo-2::GFP + SEC) 11881 bp DNA circular SYN 26-AUG-2024
DEFINITION SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology
arms.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11881)
AUTHORS Hibshman JD, Goldstein B
TITLE LEA motifs promote desiccation tolerance in vivo.
JOURNAL BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0.
PUBMED 34903234
REFERENCE 2 (bases 1 to 11881)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
date: "2021-12-14"; volume: "19(1):263. doi"; pages: "
10.1186/s12915-021-01176-0"
FEATURES Location/Qualifiers
source 1..11881
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 364..386
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 378..395
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 661..966
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind complement(2048..2076)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 2824..2844
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
CDS 3289..3309
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
protein_bind 3327..3360
/label=loxP511
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
compatible with each other, but incompatible with wild-type
loxP sites."
CDS 3370..3399
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
terminator complement(5216..5539)
/gene="tbb-2"
/label=tbb-2 terminator
/note="transcription terminator for the C. elegans tubulin
beta-2 gene"
protein_bind 7457..7481
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 7484..8509
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
CDS 7484..8509
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
protein_bind complement(8510..8534)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind 8920..8953
/label=loxP511
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
compatible with each other, but incompatible with wild-type
loxP sites."
CDS 8972..8998
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 8999..9025
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(9660..9676)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9660..9676)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9673..9695)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9684..9700
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9708..9738)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9753..9774
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9891..9908)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10062..10650)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10142..10161)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10821..11681)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11444..11463
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11682..11786)
/gene="bla"
/label=AmpR promoter
primer_bind 11854..11872
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
1021 gagcctcagg agcatcggga gcctcaggag catcgatttt atatctgagt agtatccttt
1081 gctttaaatg tccataaaac taattttata atcaataaaa caacgtttgt aaatcaactg
1141 agtttacaag tagagacatt gagggatact ttcactatgc taaagtgata atcgaccaaa
1201 taataacttc actttggtat ttattcctgt cttataaatg ttatgtatga attaaattca
1261 tatgcatatg gctcactctg acaattttta ataatcttcc agatcaatat tgactaccga
1321 tgcgggtggt cttttgcttt gaattctgct gaactttaca ccccgaacag caatgtgtgc
1381 ttcagcctaa aaaaaagtaa gtgtgttaat cagtgccccc gattcttcat tttttgcccc
1441 tctctcccgt ttcgtcggca aaagaagaga aaataaagat aagtctcaag ataggttggt
1501 aatcgctaaa gtggttgtgt ggataagagt agcaaaatgg caggaagagc actttgcgcg
1561 cacacactgt actcattgtt ctggataaaa ttctctcgtt gtttgccgtc ggatgtctgc
1621 ctctctgcat tgagccggct tcttcactat ctttagttaa cctaaaatgc cgtttctttt
1681 ctcgtatccc cactatcccg ttgaggttct ctgctctctt cgctccctac cgccagcgag
1741 caactatccg tgggggcgcc ttgctcggaa gatggggggg aagaaagaag atttttgcta
1801 tttgcacttg agaaagagac ttttcctgcg tcgatggtta gagaacagtg tgcagacact
1861 tttcagctac ctagaattac aattggatat ccccgcctcc caatccaccc acccagggaa
1921 aaagaagggc tcgccgaaaa tcaaagttat ctccaggctc gcgcatccca ccgagcggtt
1981 gacttctctc caccactttt catttttacc ctcgaccatg agtaaaggag aagaactttt
2041 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
2101 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
2161 cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc
2221 tgattattta aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt
2281 ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg
2341 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt
2401 taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt
2461 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
2521 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
2581 agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta
2641 atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca
2701 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
2761 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
2821 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
2881 caaatagatc aactgctcta attccatctc gtttctctct tttctttcaa tctctttacc
2941 cgtttctctt cccctacgtc ccccaaacct accactgcac agtaacacat acacattcaa
3001 cgtgtcacaa taaaagccca tcaacacctt ctctgttgtt ttttttcttc aaatgcattt
3061 ttttcacttt gtttaataat ttttacgcat cggatttgta ggatagtgga aatttgtcct
3121 tcaatgcttc aattgattcg acacttctca atatttttga tcaataaacc attgattttc
3181 tatttagaag aagatcactg taaataacac caaaaacatc acagcaaaaa aactcccact
3241 caattatatt actgttttta ttgaaactag aataaatttg aaaccggtga gaatctgtac
3301 tttcaatccg gaaaggtaag tttaaaataa cttcgtataa tgtatactat acgaagttat
3361 tttcagggag agcaaaagct tatctccgag gaggacctct aaggatgatc gacgccaacg
3421 tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat
3481 tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt
3541 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca
3601 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg
3661 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat
3721 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaac
3781 tagctgccct cgattgtcta tcttccattc tcaccaagtt tcaacaactt tattcagtca
3841 ttcttctctg gttcatttcc caaccaatct tcccgaataa agcccatctg tcctttcggc
3901 cacattcctc acctacatcc gtactcctta tctcccgttc ttatccagtc ttttatcgtc
3961 tctgcgtctc gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa aacttgaaaa
4021 cttgcggaaa gatttcctaa taaaaggaga cgaatccggt aggaaatcga caagtctctg
4081 cttctcgaag actaggtgag ttacatttct ttggccgaat tattttgaaa tcctaaattt
4141 gataatttca gagatgtctg taaaacttgc gtgttatgtg acggcttcgg tcactgtcgc
4201 cactcttatg gtatgcttca tgaccatgtc aaccatctac tcggaggttg atggattcag
4261 agagaagctt gataccgaga tgaatgtgtt cagagtgagt taaattatat tttgaatttt
4321 aaataatttt taattttcta gcaatctacc aatggattgt ggaaggacat agttgtcatc
4381 ggaagatcta gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac cccaactcca
4441 catgctgatg gatccccatc tgctccacca ggtcaaccac cagcagttcc accagtcttc
4501 aaccagccaa agactccaaa tggagccaat ggaaatggac caacctgcaa ctgcaatgct
4561 gataacaagt gcccagctgg accatccgga ccaaagggag ttccaggagt tccaggactc
4621 gacggagttc caggacttga cggtgttcca ggagttggag ctgatgatat cgctccacaa
4681 cgcgagtctg tcggatgctt cacttgccca caaggaccag ttggaccacc aggagctctt
4741 ggaagaccag gaccacgtgg acttccagga ccaagaggac aaaatggaaa cccaggaaga
4801 gatggacaac caggacatcc aggagagcaa ggatcatccg gtcaaatcgg aaagatcgga
4861 gagccaggac caccaggaga gaagggacgc gacgccgagc atccaatcgg aagaccagga
4921 ccaaagggac caagaggaga tcaaggacca acaggaccag ctggacagaa cggtcttcac
4981 ggaccaccag gagagccagg aaccgttgga ccagaaggac catctggaaa gcaaggacgt
5041 caaggaccag acggaaccca gggagagact ggaccagacg gaagaccagg aaaggatgcc
5101 gagtactgcc agtgcccaga caagtctcca ccatcagagg ctgtcaacgc caaccgtgga
5161 tacagaaata tctaaattgt tggtgttttc taataaaata tttgagattc agtgagactt
5221 ttttcttggc ggcacaataa agtttcctct ttttgttggg tcactcaacg aacacaatac
5281 aaatatttga gcgctcgcat ttttctcaac ctttcgggtt taaagcactt caaaatggaa
5341 ttgaagagag attgtgaaag tacatcagac agaatcacat tttgatagca ttcacttcac
5401 tcagatgcaa acttttcagt gactataaaa agtcataaaa taataacatt aaaaaagcaa
5461 ataaattagc gagagaattt tttgacaaaa agaaagaaga gtgatagaga agaagggaat
5521 gcttgaaagg attttgcatt tatcttaatc accgtcttcg agaagacgaa ccatggcgcc
5581 agtttcagaa tccaagtttc taatatagtt catcacaatg ttgacgttgg tccaacctcc
5641 ggcttgcata atctctggga tggaaactcc agcgcgagcc atgtcacggg cagctccgac
5701 acgggcggaa tgtccagacc aagccaagta acgttgtccg gaatcatcct tggctccgta
5761 gatgagacgg tgggtagctt cgaagattcc tgaaaattta ttttcagctt attcttagct
5821 aatatgttgt ttaaacttac cttcaagagc tcgagtcgag agttgtgatg ttgcactcgg
5881 ggcagcaact ccgttcttgc gaacacgaca gaacaggtag ttgtttggat catcggcgac
5941 tccggaaacc gaaatccagc gctcaacgag cttggtaact ccaagggaga gcgctttttc
6001 gactccggca gtcgagacca gcgtttttgt acgtccgatg tggatgagca ttcttcctcc
6061 gtctgtgcga ctaatatcct tgacgcgaat gcgggcaatt tcggcgatac ggagaagtgt
6121 gttgtaagca attccgagga aggcgaggtt tcggatgtcc tggcaacgat cggaattttc
6181 cataagagaa cggacctgat cgaagtcggt tctgaaaatt tcgtcaagtc ttaggattga
6241 atacattaat atttaaactt acctttcgaa ggcgagggct tgcttggctc tctctccagc
6301 atcaacattt tccttgcgga tacgtctcat tacgagagat acggcattag aatctgatgg
6361 gcgtggaagt ccagatcggc gatggagcat attgagctgt ccgagatgtt gttggatcgt
6421 cttcaccgcc aatccacgag cttggaggta gagcaagtag tcacgaacgt cttctggctc
6481 ggctgggaac cacttgcggt tgttaagctt gcaccaggca gcccaagaac ggcacactga
6541 gagaagcatc ttccaggtgt gctcggagaa ggcttgtctg tcacggaaca tatccatgag
6601 gttcttgcga acttcatcac ttgtcgcgtc aactggaagt gctggaagat tttgatgaac
6661 agtgagaaga tttgacatat tttcgaagtt ttttagatgc actagaacaa agcgtgttgg
6721 cttcctctga gcccgctttc cttatatacc cgcattctgc agccttacag aatgttctag
6781 aaggtcctag atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga
6841 aaatgtatct gggtctcttt attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc
6901 cctccttttg caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac
6961 cgttgaaaat aaataccgaa caacatttgc tctaattgtg aaattagaaa tcttcaaact
7021 ataatcatgt cactttacca ctatttccgt ccagctcaac gttccgtttt tggtgatcat
7081 ttttgctttc gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc aagccagcag
7141 ccaaattcgt tgactgagta ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa
7201 tgcatcggga aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa
7261 ttttgctatg ttttcaatta aaaaatatgt tattcaactg tttctatgag gaaaataagg
7321 ctttgcatgt aattttctta ttcagcataa tttttaatta atttgaattt tctgtcctaa
7381 cgtttatttt gttttcttgg ttatgactga tctgaaatta atttttgaat tttaaggtaa
7441 tatgtcaggc ggtgccgcaa gtttgtacaa aaaagcaggc tccatgaaaa agcctgaact
7501 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
7561 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
7621 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
7681 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
7741 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
7801 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
7861 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
7921 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
7981 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
8041 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
8101 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
8161 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
8221 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
8281 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
8341 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
8401 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
8461 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga cccagctttc
8521 ttgtacaaag tgggtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
8581 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
8641 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
8701 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
8761 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
8821 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcct
8881 tatcatatgt tacgtttcag tttatgaccg caatttttaa taacttcgta taatgtatac
8941 tatacgaagt tattttcagg gagccggctc ttatccatac gatgtcccag attacgctta
9001 cccatatgac gttccagact atgccggagg actagttcga cctgcagact ggctgtgtat
9061 aagggagcct gacatttaga ttccccagaa cataaggttg atagcgttct tgatatcgtt
9121 ctcacggtga gaaaggtcgg caacctcctc tccgatgacg gaaactggaa cggaagccat
9181 gtcggtggtc atcatacgcc aagactcgtc tccgatgtga acaactgggt agagctcacg
9241 tggaaccttg tcggaaagaa gacgggcgga agcaagtggg ataaccatac ggcgtcctgg
9301 ggtatcgatg atgtcggatt ggacgtcgac gaaaagacgg tagcgactct ctcttttata
9361 agtgtaaacc ttgaattgca tttcaccagc ccctgttctc gtcggcaaaa gagccgttca
9421 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
9481 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cgaaccggag atattgacat
9541 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
9601 catagcatac ctctttttga catacttcgg gttcctagga aatcgatacc ggtaacatgg
9661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
9721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
9781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
9841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
9901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
9961 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
10021 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
10081 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
10141 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
10201 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
10261 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
10321 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
10381 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
10441 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
10501 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
10561 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
10621 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
10681 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
10741 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
10801 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
10861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
10921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
10981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
11041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
11101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
11161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
11221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
11281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
11341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
11401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
11461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
11521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
11581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
11641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
11701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
11761 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
11821 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
11881 c
//
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pDD317 (Pmyo-2::GFP + SEC)质粒载体应用举例
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